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KMID : 0613820110210040549
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Journal of Life Science
2011 Volume.21 No. 4 p.549 ~ p.553
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Novel Method for Urinary 1-Hydroxypyrene Measurement Using Molecular Imprinting
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Yim Dong-Hyuk
Moon Sun-In
Chou Young-Sook
Park Hee-jin
Kim Dae-Seon
Lee Chul-Ho
Kim Yong-Dae
Kim Heon
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Abstract
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This study was performed to determine whether or not urinary 1-hydroxypyrene (1-OHP) levels can be accurately detected by our 1-OHP-detecting TiO2-Bead?HPLC assay that we developed based on the molecular imprinting method. Our method showed a variation coefficient of 4.97% and a between-day variation coefficient of 4.43%, suggesting that this may be a very stable method. In addition, the recovery rate of 1-OHP from a mixture of 1-OHP and similar substances using our TiO2-Bead-HPLC method was estimated to be 105.6%. The correlation coefficient between the conventional enzyme-HPLC method and this new method was 0.74 (p＜0.01) when the urine samples were tested. Based on this result, it is conceivable that our method could be a useful technique for measuring urinary 1-OHP levels. Moreover, our method has some advantages of being easier and less expensive than the conventional method. The results of this study suggest that our method can facilitate the development of a urine 1-OHP sensor using TiO₂-coating beads and that development of beads by molecular imprinting can be applied to analysis of chemicals other than 1-OHP.
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